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Медико-биологический
информационный портал
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Vol. 16, Art. 37 (pp. 383-392) | 2015
Assessment of DNA damage in peripheral blood cells of people occupationally exposed to heavy metals
Tomilin N.V., Petrov A. N., Filko O. A., Khrabrova A. V., Solovyeva N. E.,
Ivanova T. M., Shestova G. V., Sizova K. V., Rutkovsky G. V., Glushkov R. K.,Ivanenko A. A., Solovyev N. D. «Institute of Toxicology» of Federal Medico-Biological Agency
Brief summary
The alkaline gel-electrophoresis and micronucleus assay showed that the participants of exposed group had higher DNA damage of leucocyte’s nuclei and higher micronuclei frequency into reticulocytes than control group. The whole blood concentration of manganese was increasing too. Blood manganese concentration was negative correlated with DNA damage. It seems that manganese protects nuclear DNA from damage. It seems that at low concentration manganese was involved in antioxidant activity but at high concentration it was capable of to induce DNA damage in human leucocytes. Evidently genotoxicity of welding fumes is determined by other metals (lead, cobalt, nickel or aluminium), which are in welding fumes. It might be their combined action. Key words genotoxicity, leucocytes, reticulocytes, alkaline gel-electrophoresis, micronucleus assay, manganese (The article in PDF format. For preview need Adobe Acrobat Reader)  Open article in new window
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