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Vol. 15, Art. 34 (pp. 404-412)    |    2014       

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The variable positions аnd regions of hemoglobin β-chain aminoacid sequences of the carnivora mammalia

Kostetsky P.V.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS

Brief summary

The hemoglobin β-chains (Hbβ) aminoacid (АА) sequences of 16 Carnivora species (10 Caniformia and 6 Feliformia) were “group-compared” to locate the variable positions and regions of homologous amino acid sequences. It was found that this group of 16 Hbβ-sequences has 35 variable position, but the pairwise comparison gives maximum 26 AA-changes. Among 35 variable positions there are two separate sets of 12 and 10 items which belong to Caniformia or Feliformia, and are responsible for the species variety of Hbβ-molecules. Most of the variable positions are located in four variable regions and occupied positions 1-17, 51-59, 74-85 and 121-131. But the location and peculiarities of variable regions of Caniformia and Feliformia (two suborders of Carnivora) Hbβ-sequences are very different. The 51-59 region is variable (and active during evolution ) only for Caniformia and the 74-85, 121-131 regions are variable only for Feliformia Hbβ-sequences. Moreover there is the possibility to predict the taxonspecific peptide structure for the group of Caniformia Hbβ-sequences – 78-85 NLDNLKGT. This fragment is constant for 10 Caniformia Hbβ-sequences but each of 6 Feliformia Hbβ-sequences contains three AA-changes. Using the great number of artificial homologous AA-sequences - randomly permuted columns of Caniformia 10 Hbβ-sequences twodimensional array - was possible to show that the presence of variable regions is not accidental. The probability of the by chance appearance of the region with 11 variable positions among 17 position is low (confidence level 5%). Moreover the combined presence of such variable region and the second 51-59 region with 6 variable positions is also not accidental (p>99%). The confidence levels of Carnivora (р~93%) and Caniformia (р>99%) Hbβ-sequences demonstrate the high linear irregularity of the variable positions in Hbβ-molecules.


Key words

variability profile, homologous sequences, taxonspecific peptide, species variety, Monte-Carlo

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