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Медико-биологический
информационный портал
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для специалистов |
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Vol. 26, Art. 11 (pp. 288-303) | 2025
Experimental evaluation of the efficiency of using allogeneic fibroblast culture in the treatment of deep burn wounds
Kostyakova A.V.1, Kostyakov D.V.1,2, Zinoviev E.V.1,3,4, Soloshenko V.V.1, Krylov P.K.1, Semiglazov A.V.1, Vegera D.S.1, Vasilyeva A .G.3
1 Saint Petersburg I.I. Dzhanelidze Research Institute of Emergency Medicine, 192242, Russia, St. Petersburg, Budapestskaya st., 3, lit. A 2 St Petersburg University, Saint Petersburg State University, 199034, St. Petersburg, Universitetskaya embankment, 7-9 3 Saint-Petersburg State Pediatric Medical University, 194100, St. Petersburg, Litovskaya street, 2 4 S. M. Kirov Military Medical Academy, 194044, St. Petersburg, st. Academician Lebedev, 6
Brief summary
The current level of scientific development has allowed us to obtain an in-depth understanding of the mechanisms of wound defect reparation, including burns, as well as the cell lines involved. This has expanded the capabilities of regenerative medicine through targeted selective use of certain cell-based populations to restore damaged soft tissues. However, there are currently no generally accepted indications and criteria for selecting a cellular product. In order to solve this problem, a comprehensive study of biotechnological methods for restoring the skin in burn victims was conducted, one of the stages of which is this work. Objective of the study: to experimentally evaluate the effectiveness of the application of a cellular product based on allogeneic fibroblasts in the treatment of a deep burn wound in animals. Materials and methods. The experimental study was performed using 30 small laboratory animals that were modeled for a deep skin burn. All rodents were divided into two equal groups based on the selected wound management method: no treatment (control) and application of cellular products based on allogeneic animal fibroblasts. The study included planimetry, smear impressions from the wound surface, and wound biopsy specimens. Statistical data processing was performed using generally accepted variation statistics methods. The alternative hypothesis was accepted at p<0.05. Results. It was found that allogeneic fibroblast transplantation accelerated the recovery of experimental wounds by 85.1% (p<0.05) as early as the 21st day of treatment compared to the group of animals without treatment. Connective tissue thickness and the number of newly formed vessels increased by 2 times (p<0.05) and 2.8 times (p<0.05), respectively. It is noted that the application of allofibroblasts optimizes the course of the wound process by reducing the concentration of inflammatory cells by 52.2% (p<0.05) and increasing the population responsible for reparative regeneration by 3.4 times (p<0.05). This is confirmed by the results of morphological studies of wound biopsies, indicating the formation of a more mature and highly differentiated epithelium. Conclusion. The data obtained allow us to conclude that the use of allogeneic fibroblasts on the developed skin burn model reliably accelerates reparative regeneration and ensures faster restoration of the wound defect. At the same time, optimization of the course of the wound process is noted, reflected in a decrease in the concentration of inflammatory cells and an increase in the number of fibroblasts and fibrocytes. The results of the experimental study indicate the promise of the analyzed method for treating burn injuries and the need for further, including clinical, study. Key words skin burn, experimental wound, cellular products, fibroblasts, biotechnology (The article in PDF format. For preview need Adobe Acrobat Reader)  Open article in new window
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