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Медико-биологический
информационный портал
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для специалистов |
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Vol. 25, Art. 20 (pp. 352-363) | 2024
Platelet microparticle measurement in the context of safety assessment of platelet concentrates
Lastochkina D.V., Kasyanov A.D., Grishina G.V., Golovanova I.S., Smirnova O.A., Matvienko O.Y., Bessmeltsev S.S.
Russian research institute of hematology and transfusiology St. Petersburg, Russia
Brief summary
Introduction. Transfusion therapy using platelet concentrates is an important clinical option in the treatment of hematologic, gynecologic, surgical and oncologic patients. Due to the increased need for the use of this blood component, the assessment of its quality and safety is given increased attention. The aim of the work. To analyze quantitative changes in cellular composition and platelet microparticles in apheresis concentrate of platelets on storage time. Materials and Methods. The object of the study was apheresis concentrates of platelets prepared on plasma and SSP+ supplement solution. The number of platelets on storage terms was determined using hematological analyzer Medonic M20 (Sweden). The number of platelet microparticles was recorded on a CytoFLEX flow cytofluorimeter (Beckman Coulter, USA) by side scattering of violet laser radiation. Results. It was revealed that the number of platelets did not significantly change during storage, whereas the level of microparticles significantly increased both in platelet concentrates prepared on plasma and in platelet concentrates in supplement solution. Conclusion. The possibility of differentiation of platelet concentrates by the level of activation will contribute to the safety of the conducted transfusion therapy and rational use of the demanded blood component. Key words platelet concentrate; microparticles; flow cytometry; light scattering; refractoriness (The article in PDF format. For preview need Adobe Acrobat Reader)  Open article in new window
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