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Vol. 25, Art. 30 (pp. 566-581)    |    2024       

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Identification of a new hla-b allele by nanopore sequencing on the platform of the portable nanopore sequencer "nanoporus"

Ermakov AM1,2*, Blagodatski AS2, Filippova KO1,2, Rudik DV3.

1Nanoporus LLC
2Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
3JSC «BioChemMack Diagnostics»

Brief summary

. High-resolution typing of the human leukocyte antigen (HLA) locus is an important diagnostic procedure required for organ transplantation and hematopoietic stem cell transplantation (HSCT). The latter is required for the treatment of a number of malignant and non-malignant diseases. The introduction of platforms that allow high-resolution HLA typing is an important biomedical task. Objective of the study: to perform HLA typing of patients using a high-throughput nanopore sequencing method. Materials and methods. The DNA of two patients, isolated and purified by standard methods, was amplified using a reagent kit for 11-locus multiplex amplification of HLA genes, the resulting libraries were sequenced using a “Nanoporus” nanopore sequencer. Results and conclusion. We performed HLA typing of two patients using rapid and high-throughput nanopore sequencing on the new Nanoporus platform and demonstrated its good applicability for this objective. We also identified a putative new allele for HLA-B in the DNA sample of one of the two patients.


Key words

HLA, genotyping, nanopore sequencing, NGS, polymorphism

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