Since 1965 , I have practiced the basic researches and applied the techniques of "Cryomedicine" to prevent and to treat human diseases. My clinical experiences of the use of biological human products cryopreserved are as follows:
Biological Products Number of Units Number of Patients
Blood: Red cells 18,713 1,845
Platelets 896 99
Stem Cells: Marrow 338 266
Tissues: Bone 165 98
Skin 28 16
Vein 15 2
On the base of those experiences and of cryosurgery, I like to explain the following items of Cryomedicine

Contents:
1. Cryomedicine in the 20th Century
1) Joule-Thomson effect and liquid gas
2) What is blood? Blood cells, stem cells, and plasma.
3) Structure and function of biological membrane
4) Water, solute, solvent, ice crystals, and Tamman's phenomenon
5) Cryoprotection: colligative and kinetic cryoprotection, vitrification and Griffith's flaw, relaxation time of cell suspended solution at the destined cryo-temperature
6) Cryomicroscopic observation of cell, tissue and organs under subzero-temperature

2. Cryopreservation of cell, tissue and organ for clinical uses
1.. Cryopreservation of red cells. How long can we and should we cryopreserve blood cells in the frozen state?
2.. Clinical merits of transfusion of thawed red cells
3.. Cryopreservation of platelets
4.. Cryopreservation of lymphocyte and granulocyte
5.. Transplantation of bone marrow, cord, and peripheral stem and progenitor cells, which are cryopreserved.
6.. Transplantation of skin, bone and vessel cryopreserved

3. Cryosurgery.
1.. Mechanism of cryosurgery: microcirculatory failure and necrosis,
2.. High and low immune responders for cryosurgery: humoral and cellular
3.. Potential role of apoptosis provoked by cryosurgery,
4. Cryomedicine in the 21st Century

1) Cost-benefit Cryomedicine and the new energy resources
2) Preservation of beneficial genes,
3) Interdisciplinary treatment of congenital, malignant, and infectious diseases using Cryomedicine

В кн.: Достижения криомедицины. Санкт-Петербург, 2001, С.114-115.