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 УЧРЕДИТЕЛИ:
Институт теоретической и экспериментальной биофизики Российской академии наук.

ООО "ИЦ КОМКОН"

ФГБУН "Институт токсикологии" ФМБА России




Адрес редакции и реквизиты

192012, Санкт-Петербург, ул.Бабушкина, д.82 к.2, литера А, кв.378

Свидетельство о регистрации электронного периодического издания ЭЛ № ФС 77-37726 от 13.10.2009
Выдано - Роскомнадзор

ISSN 1999-6314

Российская поисковая система
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«
Vol. 16, Art. 64 (pp. 700-716)    |    2015       
»

The changes of intracellular potentials and ionic currents in neurons in the extra-and intracellular application of mercury acetate
Vislobokov A.I., Malov A.M., Orlov V.I.



Brief summary

The effect of mercury acetate (MA) in 0.1 µM, 1.0 µM, 10 µM, 100 µM and 1000 µM concentrations on the identified intact and isolated neurons of mollusks Planorbarius corneus and Lymnaea stagnalis have been studied using a microelectrode technique and the voltage-clamp method. MA at concentrations from 0.1 to 10 μM (after 3 – 5 min of its action beginning) slightly (by 1 – 2 mV) decreased the membrane rest potential in intact neurons. The trend to depolarization and increase of action potential (AP) frequency was observed. But MA at concentrations of 100–1000 μM caused the expressed and irreversible depolarization to the level of -35 – -40 mV that was accompanied with the cessation of AP generation and neuron damage. The changes of ionic currents under the MA influence were more expressed even at lesser concentrations than the biopotentials changes. Already under the MA effect at concentration of 0.1 μM the amplitude of the total inward sodium-calcium currents and the outward potassium currents was decreased inselectively and irreversibly and the suppression of currents was intensified up to zero at 1000 µM concentration. The changes came rapidly (lesser than during 1 min). The kinetics of ionic currents was not changed. The MA at concentration of 100 μM in the intracellular application on the neurons did not suppress the ionic currents, that is it was uneffective.


Key words

neurons of mollusks, mercury acetate, membrane rest potential, action potential, impulse activity, ionic currents.





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