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Vol. 16, Art. 47 (pp. 496-506)    |    2015       

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Stepanov A.A.1, Korotaev E.V.1, Bessmeltsev S.S.2, Rabinovich V.I.1, Ponomarev S.A.1, Khoreva O.E.3

1 Ugra research Institute of cellular technologies with stem cell Bank, Khanty-Mansiysk, Russia str. Mira 127-B, tel./fax (3467) 32-87-46, e-mail: glagolmail@gmail.com
2 Russian Research Institute of Hematology and Transfusiology, Saint-Petersburg
3BU "Surgut district Hospital", Surgut

Brief summary

The concentration of nucleated cells in the peripheral blood apheresis product, - highly variable parameter (46 - 370 × 10 leukocytes/liter), which reduces the safety of stem cells in addition to the graft dimethyl sulfoxide. It registered 16% of clinical cases where the safety of stem cells was less than 50% of the harvested dose. In these cases leukocytosis was >150 × 10 leukocytes/liter. We have developed a method for calculating the amount of autologous blood plasma needed to dilute the graft to reduce leukocytosis below 150 × 10 leucocytes/liter. Testing method showed the ability to significantly (p <0,05) increase in the safety of GSK cryopreservation.


Key words

apheresis, autologous transplantation, cryopreservation, cryoprotector, hematopoietic stem cells.

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Журнал основан 16 ноября 2000г.
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