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Медико-биологический
информационный портал
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Vol. 15, Art. 62 (pp. 779-788) | 2014
Optimization of enzymatic cleavage of peptide and protein drugs using specific proteases
Berizovskaya E.I.1, Ihalaynen A.A.1, Antohin A.M.1, Taranchenko V.F.1, Goncharov V.M.1, Mitrofanov D.A.1, Aksenov A.V.1, Rodin I.A.2, Shpigun O.A. 2
1 Federal State Unitary Enterprise "Scientific Center "The Signal", 107014, Moscow, Bolshaya Olen’a, 8. Tel. (495) 964-97-01, E-mail: eiberizovskaya@rambler.ru. 2 Federal State Educational Institution of higher professional education "Moscow State University with them. M.V. Lomonosov", Department of Chemistry, 119991, Moscow, GSP-1, Leninskie gori, 1, s.3. Tel. (495) 939-16-71.
Brief summary
Results of peptide and protein drugs enzymatic degradation study with different specific proteases – trypsin, Glu-C, Asp-N, Lys-C, Arg-C are reported. Recombinant human insulin and human chorionic gonadotropin had taken as representative example. Influence of pH, enzyme/substrate ratio, incubation temperature and incubation time was studied, optimal conditions for proteolysis of acting components was obtained. Key words peptides, recombinant human insulin, human chorionic gonadotropin, enzymatic digestion, specific proteases, high performance liquid chromatography. (The article in PDF format. For preview need Adobe Acrobat Reader)  Open article in new window
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