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Vol. 15, Art. 12 (pp. 117-133) | 2014
Influence of perfluorocarbon emulsion on morphofunction parameters of eritrocytes and leukocytes in vitro
Volkova S.D., Kuznetsova I.N., Bessmeltsev S.S.
Russian Research Institute of Hematology and Transfusiology, St. Petersburg
Brief summary
The influence of perfluorocarbon (PFC) emulsion on morpho-function parameters of erythrocytes and leukocytes (neutrophiles) has been studied. PFC emulsions was prepared by heigh pressure homogenisator using, natural phospholipids as surfactants. The mixtures of blood/emulsion with different relations of components was incubated at +37oС during 60 min. The erythrocyte morphology, function of neutrophiles was depended on emulsion dose in blood. It was shown that with relations of blood/emulsion = 100/1 ч10/1 discoid form of erythrocytes, bactericide and phagocyte function of neutrophiles was not changed. But metabolic activity of neutrophiles (according NBT-test) was increased. With the Increasing of emulsion content in blood (relations of blood/emulsion 10 : 2 ч10 : 5) gradual increasing of echinocyte number, NBT - test decreasing and non - completion phagocytos were received. Key words emulsions, perfluorocаrbon, erythrocytes, echinocytes, neutrophiles. (The article in PDF format. For preview need Adobe Acrobat Reader)  Open article in new window
Reference list 1. Lowe K.C. Second-generation perfluorocarbon emulsion blood substitutes // Art. Cells, Blood Substit., Immobil. Biotech. – 2000. – V. 28. – N 1. – P. 25-28. 2. Kyznecova I.N. Emylsii perftoryglerodov. Stabilnostinvitroiinvivo (obzor) // Him.-farm. jyrn. – 2003. – T.37.-№ 8. – S.20-25. 3.Vorobyov S.I. Perftoryglerodnie emylsii I II pokoleniya // Him.-farm. jyrn. – 2009. – T.43.-4.–S.30-40. 4. Kovelenov F.Y., Mikhal'tsov A.N., Malkov F.N. Perftoran as a means modulating the functional activity of liver macrophages // Bull. Exp. Boil. Med. - 2002. – 134(6): R. 551-553. 5. Spahn D.R., van Brempt R., Theilmeier G. et al. Perflubron emulsion delays blood transfusions in orthopedic surgery // Anesthesiology. - 1999. –91(5). - P. 1195 – 1208. 6. Spahn D.R., Waschke R.F., Standl T. et al. Use of Perflubron Emulsion to Decrease Allogenic Blood Transfusion in High-blood-loss Non-Cardiac Surgery (Result of a European Phase 3 Study) // Anesthesiology. - 2002. – 97(6). - P. 1338– 1349. 7. Moiseenko O.M., Srednyakov V.A., Vorobev S.I. Tehnologiya primeneniya perftorana pri lechenii oftalmopatologii // Oftalmohiryrgiya.-2007.-№2.-S.46-49. 8. Kyznecova I.N. Vliyanie emylsii ftororganicheskih soedinenii na parametri kislorodnogo obmena eritrocitov donorskoi krovi invitro // Himiko-farmacevt. jyrn. – 1980.-№ 7.-S.9-12. 9. Moroz V.V., Kozlova E.K., Bogyshevich M.S. i dr. Vliyanie perftorana na modificirovannie elektricheskimi impylsami membrani eritrocitov // Obsh.reanimatologiya.-2005.-№ 1/3 – S.5-10. 10. Dyrnovo E.A., Bespalova N.A., Myhina I.V. Vliyanie podslizistogo vvedeniya perftorana na aktivnost neitrofilov perifericheskoi krovi (eksperimentalnoe issledovanie) // Obsh.reanimatologiya.- 2007, T. III.- № 3/1. – S.34-37. 11. Tsong-Long Hwang, Chia-Lang Fang, Saleh A. al-Suwayeh, et al. Activated human neutrophil response to perfluorocarbon nanobubbles: Oxygen-dependent and –independent cytotoxic responses // Toxicology Letters. – 203 (2011). – P. 172 – 180. 12. Shehtman D.G., Safronova V.G., Gabdylhakova A.G. i dr. Vliyanie emylsii perftoryglerodov na fynkcionirovanie fermentnih sistem, otvetstvennih za generaciu aktivnih form kisloroda v neitrofilah // Biofizika, - 2003.- T. 48. – vip.4. – S. 666-672. 13. Vishneveckii F.E., Golybev A.M., Beloyarcev F.F. Sostoyanie sistemi mononyklearnih fagocitov v ysloviyah primeneniya emylsii perftororganicheskih soedinenii // Bull. eksperim. biol. i medic. – 1987.- № 103(2).-S.232-236. 14. Pshyonkina N.N. Prognozirovanie farmakokineticheskih vzaimodeistvii lekarstvennih veshestv i nanosistemi na osnove perftoryglerodnih soedinenii // Avtoref. diss…. d.b.n. – SPb. – 2012. - S. 35. 15. Aleksandrin V.V. Rannie postishemicheskie narysheniya mozgovogo krovotoka i ih korrekciya perftoranom // Obshaya reanimatologiya. -2006.-T.2, № 3. – S.12-17. 16. Aliev O.M. Izmeneniya serdechnoi gemodinamiki i koronarnogo krovotoka pri ostrom infarkte miokarda pod vliyaniem perftorana // Anesteziologiya i reanimatologiya. – 2004.-№ 3.- S.62-63. 17.Besarab D.A. Reperfyzionnie narysheniya pri kriticheskoi ishemii tonkoi kishki i ih korrekciya perftoranom // Tromboz, gemostaz i reologiya. – 2005.-№ 3.- S.71-78. 18.Soroka V.V., Nohrin S.P., Andreichyk K.A. i dr. Primenenie perftorana v lechenii bolnih s kriticheskoi ishemiei nijnih konechnostei // Angiologiya i sosydistaya hiryrgiya.- 2006. - T.12. - № 4. 19.Agapov V.S., Piminova I.A. Ocenka rezyltatov mestnogo primeneniya perftorana v kompleksnom lechenii bolnih s odontogennimi flegmonami lica i shei // Stomatologiya. – 2006.-T.85, № 5.-S.32-35. 20.Ermolenko S.V., Shapovalova N.V., Lavrentev A.A. Opit ispolzovaniya perftorana pri tyajelom sepsise // Obshaya reanimatologiya. – 2007. – T.3. - № 3/1.- S.67-70. 21.Bagnenko S.F., Lapshin V.N., Shah B.N. i dr. Principi diagnostiki i korrekcii gemodinamiki y postradavshih s sochetannoi travmoi v ostrom periode travmaticheskoi bolezni – osnova profilaktiki gipoksicheskih i reperfyzionnih povrejdenii // Efferentnaya terapiya. – 2004.-T.10, № 3.-S.60-69. 22. Kovelenov A.U., Lobzin U.V., Plyjnikov N.N. i dr. Opit primeneniya perftoryglerodnoi emylsii «Perftoran» pri lechenii bolnih s tyajelimi formami gepatita V i mikstgepatitami // Epidemiol. i infekc. bolezni.-2002.-№4.-S.33-37. 23 Metodicheskie rekomendacii «Perftoran v intensivnoi terapii krovopoteri // Dnepropetrovsk – 2004.- S. 18. 24. Kislih F.I., Komlev V.V., Frank M.M. i dr. Obosnovanie vnytritkanevogo sposoba vvedeniya perftorana prilechenii vospalitelnih zabolevanii chelustno-licevoi oblasti // Obshaya reanimatologiya. – 2007.- T. III.- № 3/1. – S.71-75. 25. Anohina E.A. Primenenie preftorana v lechenii bolnih s ostroi formoi sensonevralnoi tygoyhosti i izychenie ego vliyaniya na oksidantnyu i antioksidantnyu sistemi plazmi krovi // Rosc.otorinolaringologiya.-2007.-№1.-S.15-20. 26. Kyznecova I.N., Urchenko V.S., Kochetkova G.A. Stabilnie nanodispersii na osnove perftoryglerodov i fosfolipidov // Him.-farm. jyrn. – 2012. – T.46.-№ 2. – S.52-56. 27. Kyznecova I.N. Fynkcionalnaya aktivnost i stabilnost emylsii perftoryglerodov // Avtoref. diss…. d.b.n. – SPb. – 1999. - S. 38. 28. Kyznecova I.N. O vozdeistvii emylsii perftoryglerodov na organizm // Perftororganicheskie soedineniya v biologii i medicine. – Pyshino. 2001. – S.70-76. 29. Kyznecova I.N. Metodologicheskie osobennosti izycheniya stabilnosti emylsii perftoryglerodov // Sb. fiziologicheskaya aktivnost ftorsoderjashih soedinenii (eksperiment i klinika). – ITEB RAN. Pyshino. 1995.- S.41-50. 30. Filyov L.V., Zaharov I.I., Selivanova G.V. i dr. Citospektrofotometricheskoe issledovanie gemoglobina v eritrocitah cheloveka // Citologiya. – 1989. – T.XXXI. - № 3. – S. 336-343. 31. Instrykciya po frakcionirovaniu konservirovannoi krovi na kletochnie komponenti i plazmy. M., 1989. 32. Nagoev B.S., Shybich M.G. Znachenie testa vosstanovleniya nitrosinego tetrazoliya dlya izycheniya fynkcionalnoi aktivnosti leikocitov // Lab. delo.-1981.-№4.-S.195-198. 33. Park B.N., Fikrig S.M., Smithwick E.M. Infection and nitrobluetetrazolium reduction by neutrophils: 3 diagnostic act // Lancet.-1968.-V.2.-№ 7567.-P.532-534. 34. Pigarevskii V.E. Lizosomalno-kationnii test // Patofiziol. i eksperim. terapiya.-1975. -№3.-S.86-88. 35. Pigarevskii V.E. Zernistie leikociti i ih svoistva // M.: Medicina.- 1979. – 127 s. 36. Egorova A.P., Lebedinskii V.A. Znachenie trombocitarnoi reakcii v opredelenii invazivnosti gemoliticheskogo streptokokka // Mikrobiol. i epidemiol. – 1949. - № 5. – S.8 – 9. 37. Savchenko Z.I., Erasova V.I. Fynkcionalnoe sostoyanie neitrofilov perifericheskoi krovi y bolnih bronhialnoi astmoi // Lab. delo – 1981. - № 11. – S.661-664. 38. Mayanskii A.N., Mayanskii D.N. Ocherki o neitrofile i makrofage // Novosibirsk. Sib. otd-nie.- 1983, S. 256. 39. Volkova S.D. Informativnost NST-testa v praktike immynnogo donorstva v diagnostike bakterialnih i virysnih infekcii i kontrole effektivnosti terapii // Vestn. Slyjbi krovi Rossii.- 2008.-№3.-S.10-14. |
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