banner medline tsn
 
Медико-биологический
информационный портал
для специалистов
 
Medline.ru

СОДЕРЖАНИЕ ЖУРНАЛА:
Физико-химическая биология

Клиническая медицина

Профилактическая медицина

Медико-биологические науки


АРХИВ:

Фундаментальные исследования

Организация здравохраниения

История медицины и биологии



Последние публикации

Поиск публикаций

Articles

Архив :  2000 г.  2001 г.  2002 г. 
               2003 г.  2004 г.  2005 г. 
               2006 г.  2007 г.  2008 г. 
               2009 г.  2010 г.  2011 г. 
               2012 г.  2013 г.  2014 г. 
               2015 г.  2016 г.  2017 г. 
               2018 г.  2019 г.  2020 г.  2021 г.  2022 г.  2023 г. 

Редакционная информация:
        Опубликовать статью
        Наша статистика


 РЕДАКЦИЯ:
Главный редактор

Заместители главного редактора

Члены редколлегии
Специализированные редколлегии


 УЧРЕДИТЕЛИ:
Институт теоретической и экспериментальной биофизики Российской академии наук.

ООО "ИЦ КОМКОН"

ФГБУН "Институт токсикологии" ФМБА России




Адрес редакции и реквизиты

192012, Санкт-Петербург, ул.Бабушкина, д.82 к.2, литера А, кв.378

Свидетельство о регистрации электронного периодического издания ЭЛ № ФС 77-37726 от 13.10.2009
Выдано - Роскомнадзор

ISSN 1999-6314

Российская поисковая система
Искать: 


«
Vol. 10, Art. 2 (pp. 27-43)    |    jan 2009       
»

Novel cyto-bio - chemical method for revealing individual differencies of physiological states of the organism by complex character (pattern) of succinate dehydrogenase activity
Kondrashova M.N., Khunderyakova N.V., Zakharchenko M.V.

Institute of Theoretical and Experimental Biophysics RAS, Pushchino.



Brief summary

Novel method of succinate dehydrogenase (SDH) activity measurement in lymphocytes in smear of blood is elaborated. It is called: cyto-bio-chemical, because it combines the advantages of the standard cytochemical assay to study glass-adhered enzymes with that of modern biochemical methods for investigation of mitochondria. As a result, cytobiochemical method reveals in a smear of blood response of SDH to adrenaline in the organism which exceeds the level of quiescent state 200-400% and more instead of 20-40% activation determined by current methods. The important advantage of the method carried out by computer videomicroscopy of a great number of stained objects in a single smear is statistical validity of the each separate value obtained. The novel improvements make it possible to determine SDH activity more correctly and besides to reveal its different functional states. Novel tests are sensitive characters of individual differences in the state of the organism in norm, prepathology and diseases.


Key words

succinate dehydrogenase, cytochemistry, mitochondria, adrenaline, diagnostic set, lymphocytes





(The article in PDF format. For preview need Adobe Acrobat Reader)



Open article in new window

Reference list

1.Rustin P., Munnich A., Rotig A. Succinate dehydrogenase and human diseases: new insights into a well-known enzyme.// Eur J Human Genetic. 2002. Vol.58, №10. R. 289–291.


2. Mitochondrial Physiology (MiP). The Many Faces and Functions of an Organelle./ Ed. Erich Gnaiger – Schroecken – Vorarlberg, Austria, 2005.


3. Alaynick W.A. Nuclear receptors, mitochondria and lipid metabolism.// Mitochondrion. 2008. Vol.8, R. 329–337.


4. Syhorykov V.S., Klembovskii A.I., Nevstryeva V.V., Leonteva I.V., Belozerov U.M., Sebeleva A.I. Mitohondrialnaya priroda kardiomiopatii y detei (analiz bioptatov skeletnih mishc).// Arhiv patologii. 1997. T.5, №59. S. 12–21.


5. Vishnevskii E.L., Syhorykov V.S., Pyshkar D.U., Shabelnikova E.I., Vishnevskii A.E., Koval A.L. Vliyanie viagri na sostoyanie polisistemnogo energeticheskogo metabolizma pri stresse (eksperimentalnoe issledovanie).// Yrologiya. 2004. T. 4. S. 51–55.


6. Kondrashova M.N. Vzaimodeistvie processov pereaminirovaniya i okisleniya.// Biohimiya. 1991. T. 56. S. 388–406.


7. Korrekciya metabolicheskogo acidoza pytem podderjaniya fynkcii mitohondrii./ Maevskii E.I. i dr. – ONTI, Pyshino, 2001.


8. Kondrashova M.N. Gormonopodobnoe deistvie yantarnoi kisloti.// Vopr. Biol. Med. Farmac. Himii. 2002. №1. S. 1–7.


9. Babskii A.M., Stefankiv U.S., Kondrashova M.N., Shostakovskaya I.V. Sybstratno- gormonalnaya sistema yantarnaya kislota - kateholamini. Novie dannie. V kn.: Mitohondrii v patologii. (M.N.Kondrashova, U.G.Kaminskii, E.I.Maevskii red.) ONTI Pyshino, 2001, 14-21.


10. Hynderyakova N.V., Zaharchenko M.V., Zaharchenko A.V., Kondrashova M.N. Giperaktivaciya sykcinatdegidrogenazi v limfocitah krovi novorojdennih krisyat.// Biohimiya. 2008. T. 73, №3. S. 414–419.


11. Shostakovskaya I.V., Doliba N.M., Babskii A.M., Kondrashova M.N. Aktivaciya acetilholinom okisleniya alfa-ketoglytarata v mitohondriyah pecheni.// Ykr. Bioh. Jyrn. 1986. T. 58, №6. S. 54 –61.


12. Kondrashova M.N., Doliba N.M. Polarographic observation of substrate-level phosphorylation and its stimulation by acetylcholine.// FEBS Lett. 1989. Vol. 243. P. 153 – 155.


13. Doliba N.M., Kyrgaluk N.N., Abdyla Lokal, Shostakovskaya I.V., Kondrashova M.N. Reciproknoe sykcinaty i kateholaminam deistvie vvedennih alfa-ketoglytarata i acetilholina na okislenie sybstratov v mitohondriyah serdca i neirogymoralnii statys organizma.// Mitohondrii v patologii. ONTI Pyshino, 2001. S. 21–27.


14. Kondrashova M.N., Fedotcheva N.I., Saakyan I.R., Sirota T.V., Zaharchenko M.V., Leontev D.S., Ignatev D.A., Temnov A.V., Samohvalov V.A. Sybstratno-gormonalnaya sistema regylyacii fiziologicheskogo sostoyaniya. Ysloviya ee viyavleniya. Ispolzovanie v praktike.// Gorizonti biofiziki. ONTI; NCBI; Pyshino, 2003. S. 147–154.


15. Kondrashova M.N., Kuznetzova G.D. Succinic acid as a physiological signal molecule./ Signal molecule and behaviour. Eds. W. Winlow et. al. Manchester University Press, Manchester; New York, 1991. R. 295 –300.


16. Kondrashova M.N., Zaharchenko M.V., Samohvalov V.A., Shihlyarova A.I., Barsykova L.P., Maryanovskaya G.Ya., Garkavi L.M. Signalnoe deistvie yantarnoi kisloti i ee lechebnoe primenenie v malih dozah. // Regylyatori energeticheskogo obmena. – 12 Rossiiskii Nacionalnii kongress «Chelovek i Lekarstvo». M, 2005. S. 8–16.


17. Kondrashova M.N., Maevsky E.I., Grigorenko E.V., Babsky A.M., Doliba N.M., Saakyan I.R., Sirota T.V., Fedotcheva N.I., Samokhvalov V.A. The interaction of Krebs cycle with sympathetic and parasympathetic nervous system. Substrate-hormonal system.// International Conference on Mitochondria, from Molecular Insight to Physiology and Pathology. 40 Years of Bari Meetings. Dedicated to Ernesto Quagliariello. Bari; Italy, 2005. R. 74.


18. Maevsky E.I., Rosenfeld A.S., Grishina E.V., Peskov A.B., Kondrashova M.N. Signal, sympathetic action of succinate in experimental and clinical studies.// BBA, Suppl., 14th EBEC Short Reports. 2006. Vol. 14. P. 536–537.


19. He, W., Mlao, F.J. - P., Lin, D.C. – H., Schwandner, R.T., Wang, Z., Gao, J., Chen, J.- L., Tlan, H., and Ling, L. Citric acid cycle intermediates as ligands for orphan G-protein-coupled receptors.// Nature, 2004. Vol. 429. P. 188–193.


20. Yantarnaya kislota v medicine, pishevoi promishlennosti, selskom hozyaistve./ Pod red. M.N. Kondrashova i dr. ONTI, Pyshino, 1997.


21. Mitohondrii v patologii./ Pod red. M.N. Kondrashova i dr. – ONTI, Pyshino, 2001.


22. Regylyatori energeticheskogo obmena. Kliniko-farmakologicheskie aspekti.// Materiali simpoziymov na IX, X, XI, XII, XIII Rossiiskom Nacionalnom kongresse «Chelovek i Lekarstvo»./ Pod red. V.A. Hazanov. – M, 2002; 2003; 2004;2005; 2006.


23. Kondrashova M.N., Sirota T.V., Temnov A.V., Beloysova J.V., Petrynyaka V.V. Obratimaya organizaciya mitohondrii v associati kak faktor regylyacii dihaniya.// Biohimiya. 1997. T. 62, №6. S. 154–163.


24. Kondrashova M.N., Fedotcheva N.I., Saakyan I.R., Sirota T.V., Lyamzaev K.G., Kulikova M.V., Temnov AV. Preservation of native properties of mitochondria in rat liver homogenate.// Mitochondrion. 2001. Vol. 1. P. 249–267.


25. Zaharchenko M.V., Temnov A.V., Kondrashova M.N. Vliyanie karnozina na samoorganizaciu ansamblei mitohondrii v gomogenate pecheni krisi.// Biohimiya. 2003. T.68. S. 1226–1230.


26. Temnov A.V., Sirota T.V., Stavrovskaya I.G., Foigel A.G., Kondrashova M.N. Vliyanie syperoksida vozdyha na stryktyrnyu organizaciu i fosforiliryushee dihanie mitohondrii.// Biohimiya. 1997. T.62. S. 1272 – 1279.


27. Narcissov R.P. Mitohondrialnie bolezni. Vzglyad citohimika. M., Medicina,1999.


28. Diagnosticheskie i prognosticheskie vozmojnosti klinicheskoi citohimii. Petrichyk S.V. i dr. M. 2005.


29. Pat. № 2007143021/15(047111). Rossiya. Prioritet ot 20 noyabrya 2007. Citobiohimicheskii sposob opredeleniya aktivnosti sykcinatdegidrogenazi, okisleniya endogennoi yantarnoi kisloti, signalnogo deistviya mikromolyarnih koncentracii yantarnoi kisloti, ego primenenie dlya kolichestvennoi ocenki yrovnya adrenergicheskoi regylyacii v organizme, sreda i nabor dlya osyshestvleniya sposoba. / ITEB RAN, Kondrashova M.N., OAO DIOD, Zaharchenko M.V., Hynderyakova N.V., Maevskii E.I.


30. Kondrashova M.N., Zakharchenko M.V., Khunderyakova N.V. Preservation of the in vivo state of mitochondrial network for ex vivo physioliogical study of mitochondria.//Int J Biochem Cell Biol (2009), doi:10.1016/j.biocel.2009.04.020.


31. Hynderyakova N.V., Zaharchenko M.V., Maevskii E.I., Kondrashova M.N. Metodika opredeleniya citobiohimicheskih pokazatelei (patternov) aktivnosti sykcinatdegidrogenazi na primere obsledovaniya pacientov s pishevoi allergiei i sopytstvyushei gipertoniei.//Vestnik Novih medicinskih Tehnologii. 2009 (v pechati).



Журнал основан 16 ноября 2000г.
Выдано Министерством РФ по делам печати, телерадиовещания и средств массовых коммуникаций
(c) Перепечатка материалов сайта Medline.Ru возможна только с письменного разрешения редакции

Размещение рекламы

Rambler's Top100