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Vol. 21, Art. 21 (pp. 236-245)    |    2020       

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Determination of the gold nanoparticles optimal parameters as a matrix for the immobilization of highly purified human butyrylcholinesterase

Federal State Unitary Enterprise "Research Institute of Hygiene, Toxicology and Occupational Pathology" of Medical and Biological Agency, Russia

Brief summary

The purpose of the work is the use of gold nanoparticles having the optimal configuration as a platform for the immobilization of highly purified butyrylcholinesterase (hpBuChE). The possibilities as a matrix for the immobilization of gold nanoparticles of the following shapes and dimensions were synthesized and studied: nanospheres 15 nm and 50 nm, as well as nanorods 12 × 46 nm and 20 × 60 nm. The study of the activity of the obtained immobilized forms of highly purified butyrylcholinesterase in in vitro experiments revealed the increase in substrate activity of enzyme conjugates with gold nanospheres of 15.1 nm by 28% compared with the activity of the native enzyme. HpBuChE conjugates with gold nanoparticles with a diameter of 50 nm were less active, but also exceeded the initial form by 8.5%. The use of gold nanorods as a matrix for immobilization led to inhibition of enzyme activity.


Key words

highly purified butyrylcholinesterase, spherical gold nanoparticles, gold nanorods, activity, immobilized enzymes, conjugation.

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