The aim of this work was to study DD (dedifferentiation) / EMT (epithelial mesenchymal transition) of renal tubular epithelium in patients with IgA- nephropathy with tubulointerstitial fibrosis, as well as an analysis of their interconnections. For histopathological studies, preparations were stained with standard stains. Changes were analyzed semi-quantitatively. Immunohistochemical studies were performed using monoclonal antibodies to SMA and Vimentin (Dako). Statistical processing was carried out be using standard software packages for applied statistical analysis (Statistica 8.0 for Windows) using parametric and nonparametric statistics.
The results of a quantitative and semi-quantitative study of pathomorphological parameters of fibrosis in the group of patients with IgA- nephropathy were characterized by their increase
in the patients of ?3 to ? 5 gr., compared to others. Analysis the number myofibroblasts in tubulointerstitium (SMA ?positive?), tubular epithelium with signs of DD/EMT (Vimentin ?positive?), the relative volume of tubular epithelium and stromal area did not reveal significant differences between the groups. Although in the group ?5 the tendency to increasing was observed. A correlation analysis of patients with IgA-nephropathy revealed a connection between the quantity of Vimentin? positive? epithelial cells and the urea level (r = -0.52) in gr. ? 3, as well as the number of ?SMA positive? cells in the tubulointerstitium of the general group. In our opinion, the obtained data indicates that the DD EMT process of the renal tubule epithelium in IgAN- nephropathy occurs and is accompanied by increased activity of sclerotic processes in tubulointerstitium, profibrotic markers (myofibroblasts), as well as some changes of clinical and laboratory indicators renal dysfunction (24- hours proteinuria and urea)..
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