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Vol. 21, Art. 10 (pp. 107-118)    |    2020       

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Determination of monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine by gc-ms method

Federal State Unitary Enterprise Research and Technical Center of Radiation-Chemical Safety and Hygiene

Brief summary

A procedure was developed and tested for measuring ten metabolites of polycyclic aromatic hydrocarbons PAHs, including: 1- and 2-hydroxynaphthalene, 2-, 3-, 9-hydroxyfluorene, 1-, 2-, 3-, 4-hydroxyphenanthrene and 1-hydroxypyrene. The technique is based on the enzymatic hydrolysis of urine samples into which a solution of the corresponding isotope-labeled standards-imitators was previously introduced; solid phase extraction (SPE) of monohydroxylated metabolites from the hydrolyzate; concentrating the extract; derivatization of the sample with N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA) and its subsequent analysis using chromatography-mass spectrometry (GC/MS). The correlation coefficients of the calibration dependence were more than 0.99. The technique was tested on a cohort of volunteers.


Key words

biomonitoring, PAH metabolites, biomarker, gas chromatography-mass spectrometry (GC/MS), solid phase extraction (SPE), derivatization.

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