Effect of temperature variation and shake by long transfer on viability and clonogenic potential of hematopoietic stem cells for clinical transplantation
A. Stepanov, E. Korotaev, V. Rabinovich, E. Selivanov, L. Astahova, S. Ponomarev.
We have analyzed the effect of temperature variation and shake on viability and progenitor’s ability of hematopoietic stem cells. The result of research presents arguments for choosing of the best conditions for transportation of hematopoietic stem cells for clinical transplantation.
We have made experimental investigations of influence of temperature and shake at different long time at transportation on important characteristic of the transplantation material. We have researched processes of destruction of leukocytes by flow cytometry and progenitor’s ability of stem cells by quantitation assay colony of hematopoietic progenitors.
Results of our analysis speak about decrease progenitor’s activity of stem cells by the transportation after 6 hours, and them progenitor’s activity decrease in during 24 hours. The dynamic of decrease of progenitor’s ability of stem cells is describing at linear function and that allows to us to predict of changes of transplantation material at long transportation.
Our research has showed that destruction of leukocytes is more at temperature +4°С, than at temperature +24°С, but progenitor’s ability of stem cells has not depended from this temperature conditions. It has showed the optimum temperature of transportation of transplantation material.
Mechanical influences have not effect on progenitor’s ability of stem cells and on intensity of destruction of leukocytes.
hematopoietic stem cells, clinical transplantation, conditions of transportation of material for clinical transplantation
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