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Vol. 11, Art. 30 (pp. 362-375)    |    2010       

№ гос. регистрации: 0421000002030

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Study of stimulation of Ca²⁺ ions slow efflux from rat liver mitochondria by comuton from this organ

G.M. Elbakidze, A.G. Elbakidze, A.G. Medentsev*

Biomedical Center, Association for World Laboratory, Moscow
*- Institute of Biochemistry and Physiology of microorganisms, Pushchino, Moscow Region

Brief summary

It is known that activation of fixed macrophages - Kupffer cells - in liver by treatment of animal with endotoxin (prodigiozan) leads to accumulation of prodigiozan-dependent comuton (PDC) in liver, able to regulate Ca2+ ions metabolism in mitochondria of hepatocytes tissue-specifically. It was demonstrated that Ca2+ ions accumulation in mitochondrial matrix is necessary condition for stimulation of slow efflux of Ca2+ ions from mitochondrial matrix by PDC. It is established that stimulation of slow efflux of Ca2+ ions from matrix of liver mitochondria caused by PDC is insensitive to cyclosporine A and not accompanied by lowering of transmembrane potential as well as by high-amplitude swelling of mitochondria. The conclusion was made that in sodium free incubation media PDC possess an ability to regulate slow efflux of Ca2+ ions via Ca2+/H+ exchange mechanism, but not by opening of permeability transition pore in a small part of mitochondria in suspension.


Key words

mitochondria, hepatocytes, calcium efflux, comuton, Kupffer cells.

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