The temperature-induced micelles-to-vesicles transformation in binary mixtures dimyristoylphosphatidylcholine-sodium cholate was studied. Small unilamellar or large multilamellar vesicles were shown to form in this mixture depending on the lipid and detergent concentrations and temperature. An endothermic process was found to occur during the micelles-to-vesicle transformation. It was demonstrated that the lipid gel-to-liquid-crystal phase transition is prerequisite to the transformation.
Key words
The micelles-to-vesicles transformation, structural transitions, lipid-detergent mixtures, turbidimetry, DSC.
1. Lichtenberg D. Micelles and Liposomes // Biomembranes. Physical Aspects M. Schinitzky Ed. VCH. 1993. P. 63-95.
2. Storm G., Crommelin J.A. Liposomes: quo vadis? // Pharm. Sci. Technol. Today. 1998. V. 1, P. 19-31.
3. Carey M.C., Small D.M., Bliss C.M. Lipid digestion and absorption // Annu. Rev. Physiol. 1983. V. 45, P. 651-677.
4. Caffrey M. Membrane protein crystallization // J. Struct. Biol. 2003. V. 142, P. 108-132.
5. Prosser R.S., Evanics F., Kitevski J.L., Al-Abdul-Wahid M.S. Current applications of bicelles in NMR studies of membrane-associated amphiphiles and proteins // Biochemistry 2006. V. 45, P. 8453-8465.
6. Rigaud J.L. Membrane proteins: functional and structural studies using reconstituted proteoliposomes and 2-D crystals // Braz. J. Med. Biol. Res. 2002. V. 35, P. 753-766.
7. Garavito R.M., Ferguson-Miller S. Detergents as tools in membrane biochemistry // J. Biol. Chem. 2001. V. 276, P. 32403-32406.
9. Polozova A.I., Dubachev G.E., Simonova T.N., Barsukov L.I. Temperature-induced micellar-lamellar transformation in binary mixtures of saturated phosphatidylcholines with sodium cholate // FEBS Letters 1995. V. 358, P. 17-22.
10. Dybachev G.E., Polozova A.I., Simonova T.N., Borovyakin V.L., Demin V.V., Barsykov L.I. Elektronno-mikroskopicheskoe issledovanie perehodnih stryktyr pri termoindyciryemoi transformacii micelli- vezikyli v sisteme dimiristoilfosfatidilholin – holat natriya // Biologicheskie membrani 1996. № 13, S. 100-108.
11. Lesieur P., Kiselev M.A., Barsukov L.I., Lombardo D. Temperature-induced micelle to vesicle transition: kinetic effects in the DMPC/NaC system // J. Appl. Crystallogr. 2000. V. 33, P. 623-627.
12. Inoue T., Kawamura H., Okukado S., Shimozawa R. Characterization of molecular assemblies formed in aqueous C10E7/DPPC mixture by spin label and fluorescence probe techniques and mechanism of micelle-to-vesicle transformation // J. Colloid Interf. Sci. 1994. V. 168, P. 94-102.
13. Majhi P.R., Blume A. Temperature-induced micelle-vesicle transitions in DMPC-SDS and DMPC-DTAB mixtures studied by calorimetry and dynamic light scattering // J. Phys. Chem. B 2002. V. 106, P. 10753-10763.
14. Van Dam L., Karlsson G., Edwards K. Direct observation and characterization of DMPC/DHPC aggregates under conditions relevant for biological solution NMR // Biochem. Biophys. Acta 2004. V. 1664, P. 241-256.
15. Carion-Taravella B., Chopineau J., Ollivon M., Lesieur S. Phase behavior of mixed aqueous dispersions of DPPC and dodecyl glycosides: Aggregation states implicated in the micelle-to-vesicle transition // Langmuir 1998. V. 14, P. 3767-3777.
16. Miguel M., Eidelman O., Ollivon M., Walter A. Temperature Dependence of the Vesicle-Micelle Transition of Egg Phosphatidylcholine and Octyl Glucoside // Biochemistry 1989. V. 28, P. 8921-8928.
17. Schnitzer E., Lichtenberg D., Kozov M.M. Temperature-dependence of the solubilization of dipalmitoylphosphatidylcholine (DPPC) by the non-ionic surfactant Triton X-100, kinetic and structural aspects // Chem. Phys. Lipids 2003. V. 126, P. 55-76.
18. Bayerl T.M., Werner G., Sackmann E. Solubilization of DMPC and DPPC vesicles by detergents below their critical micellization concentration: high-sensitivity differential scanning calorimetry. Fourier transform infrared spectroscopy and freeze-fracture electron 148 microscopy reveal two interaction sites of detergents in vesicles // Biochim. Biophys. Acta 1989. V. 984, P. 214-224.
19. Inoue T., Motoyama R., Totoki M., Miyakawa K., Shimozawa R. Molecular aggregates formed in aqueuos mixtures of POE type nonionic surfactants and phosphatidylcholines // J. Colloid Interf. Sci. 1994. V. 164, P. 318-324.
20. Koynova R., Caffrey M. Phases and phase transitions of the phosphatidylcholines // Biochim. Biophys. Acta. 1998. V. 1376, P. 91-145.
21. Yi P.N., Macdonald R.C. Temperature dependence of optical properties of aqueous dispersions of phosphatidylcholine // Chem.Phys.Lipids 1973. V. 11, P. 114-134.
22. Golcov A.N., Kondrateva O.V., Barsykov L.I. Stryktyrnii perehod micelli-vezikyli v smeshannoi sisteme, sostoyashei iz dimiristoilfosfatidilholina i holata natriya // Nelineinie yavleniya v otkritih sistemah. Moskva, 1997. Vip. 8, S. 87–101.