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Vol. 24, Art. 28 (pp. 380-390)    |    2023       

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Analysis of the study of the use butyrylcholinesterase on the function of external respiration and evaluate of the protective effectiveness of the enzyme in the model of poisoning of guinea pigs with an organophosphate compound

Kryazhevskikh A.A., Subbotina S.N., Fedonyuk V.P., Kryazhevskikh A.A., Ivanov I.M.

State Scientific Research Test Institute of the military medicine Defense Ministry of the Russian Federation

Brief summary

The article presents the results of studying the effect of inhalation use of butyrylcholinesterase (BChE) on the function of external respiration in guinea pigs. It was shown that the enzyme during the first 25 minutes of inhalation contributes to a significant decrease in the minute volume of respiration, which is completely restored within 30 minutes after the end of inhalation. The effectiveness of the prophylactic use of BChE was assessed in animal three experimental groups that differed in the intensity of exposure to the toxicant. It has been shown that the prophylactic use of BChE in case of inhalation poisoning of guinea pigs with organophosphate compound (OP) - diisopropylfluorophosphate (DFP) in lethal doses can significantly reduce the severity of symptoms of poisoning and lethal outcomes. Trus, when exposed to OP at а dose of 1 LD50, BChE prevented the development of seizures and death of animal in the acute post-intoxication period. With an increase in the absorbed dose of DFF to 1 LD67, BChE therapy contributed to an increase in the time of development of the convulsive syndrome. When poisoning guinea pigs whin OP in dose of 1 LD100, the use of the enzyme made it possible to significantly increase the survival rate of animals. In addition, the prophylactic use of BChE makes it possible to prevent the development of manifestations of respiratory failure in poisoned animal. On the background of BChE therapy, such respiratory characteristics as minute respiratory volume, tidal volume, and the integral index of bronchoconstriction Penh during inhalation exposure to OPs remained at the level of initial values.


Key words

butyrylcholinesterase; diisopropylfluorophosphate; inhalation administration; respiratory function; protective effectiveness; organophosphate poisoning

Reference list

1. Pandey S., Shrestha N. Organophosphorus poisoning among acute poisoning cases presenting to the emergency department of a secondary care centree: a descriptive cross-sectional study. Journal of Nepal Medical Association. 2022; 60 (249): 435-438. http://doi.org/10.31729/jnma.7446.


2. Antidotnaya terapiya otravlenii visokotoksichnimi veshestvami v ysloviyah chrezvichainih sityaciisityaciyah / Pod red. V.D. Gladkih, S.H. Sarmanaeva, U.N. Ostapenko. M.: Kommentarii; 2014.


3. Antohin A.M., Gainyllina E.T., Taranchenko V.F., Rijikov S.B., Yavaeva D.K. Holinesterazi: stryktyra aktivnogo centra i mehanizmi vliyaniya blokatorov holinoreceptorov na skorost vzaimodeistviya s ligandami. Yspehi himii. 2010; 79 (8): 780-795. http://doi.org/10.1070/RC2010v079n08ABEH004025


4. Ivleeva N.V. Otravleniya bitovimi fosfororganicheskimi soedineniyami. Medicinskaya sestra. 2016; (5): 24-29.


5. Temchenko A.V. Fosfororganicheskie soedineniya: toksikologicheskaya harakteristika i stepen opasnosti. Mejdynarodnii stydencheskii naychnii vestnik. 2017; 3 (4): 289-291.


6. Giyanwani P.R., Zubair U., Salam O. et al. Respiratory failure following organophosphate poisoning: a literature review. Cureus. 2017; 9 (9). http://doi.org/10.7759/cureus.1651. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669523/ (data obrasheniya 01.02.2023).


7. Vengerovich N.G., Udin M.A., Nikiforov A.S. i dr. Obosnovanie vibora antioksidantov, perspektivnih dlya vvedeniya v vide aerozolei pri ingalyacionnom porajenii otravlyaushimi veshestvami. Medlain.ry. 2021; 22: 35-48; URL: http://www/medline.ru/public/art/tom22/art3.html (data obrasheniya 16.02.2023).


8. Ivanov I.M., Sybbotina S.N., Alhytova I.M. i dr. Mehanizmi naryshenii dihaniya pri otravleniyah fosfororganicheskimi soedineniyami i napravleniya korrekcii pri pomoshi bronhorasshiryaushih sredstv. Medlain.ry. 2022; 23: 185-200; URL: http://www/medline.ru/public/art/tom23/art12.html (data obrasheniya 10.02.2023).


9. Vengerovich N.G., Drachkova I.M., Udin M.A. i dr. Eksperimentalnoe obosnovanie ingalyacionnogo vvedeniya sredstv patogeneticheskoi terapii toksicheskogo sydorojnogo sindroma dlya korrekcii naryshenii fynkcii vneshnego dihaniya. Bulleten eksperimentalnoi biologii i medicini. 2018; 165 (2): 188-192.


10. Sventickaya A.M., Orlova A.B., Sybbotina S.N. i dr. Perspektivi primeneniya skavendjerov v kachestve sredstv antidotnoi terapii ostrih otravlenii veshestvami antiholinesteraznogo deistviya (obzor literatyri). Medlain.ry. 2020; 21: 452-473; URL: http://www/medline.ru/public/art/tom21/art37.html (data obrasheniya 09.02.2023).


11. Masson P., Roshy D. Kataliticheskie «biolovyshki» protiv toksicheskih efirov, alternativnii podhod dlya profilaktiki i lecheniya otravlenii. Acta Naturae. 2009; (1): 70-81.


12. Udin M.A., Chepyr S.V., Fedonuk V.P. i dr. Sravnitelnii analiz sozdaniya antidotov fosfororganicheskih otravlyaushih veshestv v Rossii i za rybejom. Voenno-medicinskii jyrnal. 2019; 340 (2): 18-26.


13. Filimonov I.V., Yankovskaya A.A., Kyjelko S.V. i dr. Issledovaniya v sfere perspektivnogo ispolzovaniya himiko-biologicheskih i medicinskih biokataliticheskih tehnologii v interesah vooryjennih sil. Vestnik voisk RBH zashiti. 2018; 2 (2): 18-50.


14. diTargiani R.C., Chandrasekaran L., Belinskaya T. et al. In search of a catalytic bioscavenger for the prophylaxis of nerve agent toxicity. Chem. Biol. Interact. 2010; 187 (1-3); 349-354. http://doi.org/10.1016/j.cbi.2010.02.021.


15. Sun W., Doctor B.P., Lenz D.E. et al. Long-term effects of human butyrylcholinesterase pretreatment followed by acute soman challenge in cynomolgus monkeys. Chem. Biol. Interact. 2008; 175 (1-3): 428-430.


16. Doctor B.P., Saxena A. Bioscavenger for protection of humans against organophosphate toxicity. Chem. Biol. Interact. 2005; 157-158: 167-171.


17. Turenkov I.N., Velikorodnaya U.I. O roli bytirilholinesterazi v patogeneze neirodegenerativnih zabolevanii: spornie aspekti. Yspehi sovremennoi biologii. 2020; 140 (5): 455-463.


18. Ilushin D.G., Ertle O.M., Bobik T.V. i dr. Rekombinantnaya bytirilholinesteraza cheloveka kak biologicheskii antidot novogo pokoleniya: razrabotka eykarioticheskoi sistemi ekspresii. Acta Naturae (rysskoyazichnaya versiya). 2013; 16 (1): 76-87.


19. Velikorodnaya U.I., Antonov V.A., Pochepcov A.Ya. i dr. Vliyanie visokochastotnoi bytirilholinesterazi cheloveka, konugirovannoi s kolloidnim zolotom, na aktivnost v-esteraz posle vozdeistviya diizopropilftorfosfata. Vestnik Volgogradskogo gosydarstvennogo medicinskogo yniversiseta. 2019; 4 (72): 69-72.


20. Saxena A., Sun W., Fedorko J.M. et al. Prophylaxis with human serum butyrylcholinesterase protects guinea pigs exposed to multiple lethal doses of soman or VX. Biochem. Pharmacol. 2011; 81 (1): 164-169. http://doi.org/10.1016/j.bcp.2010.09.007.


21. Rosenberg Y.J., Laube B., Mao L. et al. Pulmonary delivery of an aerosolized recombinant human butyrylcholinesterase pretreatment protects against aerosolized paraoxon in macaques. Chem. Biol. Interact. 2013; 203: 167-171.


22. Rosenberg Y.J., James B. Creation of a protective pulmonary bioshield against inhaled organophosphates using an aerosolized bioscavenger. Ann. N. Y. Acad. Sci. 2016; 1374 (1): 151-158. http://doi.org/10.1111/nyas.13106.


23. Kryajevskih A.A., Kryajevskih A.A., Sybbotina S.N. i dr. Vliyanie fosfororganicheskih soedinenii na fynkciu vneshnego dihaniya kris pri intratrahealnom i vnytrijelydochnom vvedenii. Formyli Farmacii. 2021; 3 (2): 60-65. http://doi.org/10,17816/phf72068


24. Bikov V.N., Sarana A.M., Drachkova I.M. i dr. Issledovanie vliyaniya antikonvylsantov na techenie dihatelnoi nedostatochnosti pri otravleniyah fosfororganicheskimi soedineniyami. Vestnik Rossiiskoi voenno- medicinskoi akademii. 2014; 1 (45): 110-115.


25. Frazer D.G., Reynolds J.S., Jackson M.C. Determining when enhanced pause (Penh) is sensitive to changes in specific airway resistance. Toxicol Environ Health. 2011; 74 (5): 287-295.