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Vol. 19, Art. 75 (pp. 1053-1064)    |    2018       

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Reason for the use Daphnia Magna Stratus for screening of chemical compounds with the properties of inhibitors of alcoholdehydrogenase

1Gorbacheva T.V., 2Bonitenko E.Y., 1Basharin V.A., 3Bonitenko K.E.

1Military Medical Academy named S.M. Kirov
2Izmerov Research Institute of Occupational Health
3Federal State Budgetary Educational Institution of Higher Education Academician I.P. Pavlov First St. Petersburg State Medical University of the Ministry of Healthcare of Russian Federation

Brief summary

Studying of a role of alcohol dehydrogenase in biotransformation of ethanol at Daphnia Magna Stratus was the aim of research. By method of pharmacological probing with use of un-competitive alcohol dehydrogenase inhibitor - amide of isovalerian acid has established that bio-transformation of ethanol in daphnia is performed by the participation of NAD-dependent alcohol dehydrogenase. The share of alcohol dehydrogenase accounts for at least 84% of the total metab-olism of ethyl alcohol in daphnia. It was concluded that Daphnia Magna Stratus could be used as a test object for screen-ing substances with the properties of alcohol dehydrogenase inhibitors.


Key words

Daphnia Magna Stratus, ethyl alcohol, alcoholdehydrogenase, metabolism, inhibitors of alcoholdehydrogenase.

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