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Институт теоретической и экспериментальной биофизики Российской академии наук.

ООО "ИЦ КОМКОН".




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199406, Санкт-Петербург, ул.Гаванская, д. 49, корп.2

ISSN 1999-6314

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«
Vol. 16, Art. 64 (pp. 700-716)    |    2015       
»

The changes of intracellular potentials and ionic currents in neurons in the extra-and intracellular application of mercury acetate
Vislobokov A.I., Malov A.M., Orlov V.I.



Brief summary

The effect of mercury acetate (MA) in 0.1 µM, 1.0 µM, 10 µM, 100 µM and 1000 µM concentrations on the identified intact and isolated neurons of mollusks Planorbarius corneus and Lymnaea stagnalis have been studied using a microelectrode technique and the voltage-clamp method. MA at concentrations from 0.1 to 10 μM (after 3 – 5 min of its action beginning) slightly (by 1 – 2 mV) decreased the membrane rest potential in intact neurons. The trend to depolarization and increase of action potential (AP) frequency was observed. But MA at concentrations of 100–1000 μM caused the expressed and irreversible depolarization to the level of -35 – -40 mV that was accompanied with the cessation of AP generation and neuron damage. The changes of ionic currents under the MA influence were more expressed even at lesser concentrations than the biopotentials changes. Already under the MA effect at concentration of 0.1 μM the amplitude of the total inward sodium-calcium currents and the outward potassium currents was decreased inselectively and irreversibly and the suppression of currents was intensified up to zero at 1000 µM concentration. The changes came rapidly (lesser than during 1 min). The kinetics of ionic currents was not changed. The MA at concentration of 100 μM in the intracellular application on the neurons did not suppress the ionic currents, that is it was uneffective.


Key words

neurons of mollusks, mercury acetate, membrane rest potential, action potential, impulse activity, ionic currents.





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Reference list

1. Yuan Y. Methylmercury: a potential environmental risk factor contributing to epileptogenesis // Neurotoxicology. – 2012. – Vol. 33 (1). – P. 119–126.


2. Atchison W.D. Effects of toxic environmental contaminants on voltagegated calcium channel function: from past to present // J. Bioenerg. Biomembr. – 2003. – Vol. 35. – P. 507–532.


3. Castoldi A.F., Coccini T., Ceccatelli S., Manzo L. Neurotoxicity and molecular effects of methylmercury // Brain Res, Bull. – 2001. – Vol. 55 (2). – P. 197–203.


4. Huang C.F., Hsu C.J., Liu S.H., Lin-Shiau S.Y. Neurotoxicological mechanism of methylmercury induced by low-dose and long-term exposure in mice: oxidative stress and down-regulated Na+/K(+)-ATPase involved // Toxicol. Lett. – 2008. – Vol. 176 (3). – P. 188–197.


5. Pekel M., Platt B., Busselberg D. Mercury (Hg2+) decreases voltage-gated calcium channel currents in rat DRG and Aplysia neurons // Brain Res. – 1993. – Vol. 632. – P. 121–126.


6. Peng S., Hajela R.K., Atchison W.D. Effects of methylmercury on human neuronal L-type calcium channels transiently expressed in human embryonic kidney cells (HEK-293) // J. Pharmacol. Exp. Ther. – 2002. – Vol. 302. – P. 424–432.


7. Yuan Y., Atchison W.D. Methylmercury induces a spontaneous, transient slow inward chloride current in Purkinje cells of rat cerebellar slices // J. Pharmacol. Exp. Ther. – 2005. – Vol. 313. – P. 751–764.


8. Magour S., Maser H., Greim H. The effect of mercury chloride and methyl mercury on brain microsomal Na-K-ATPase after partial delipidisation with Lubrol // Pharmacol. Toxicol. – 1987. – Vol. 60. – P. 184–186.


9. Huang C.F., Hsu C.J., Liu S.H., Lin-Shiau S.Y. Neurotoxicological mechanism of methylmercury induced by low-dose and long-term exposure in mice: oxidative stress and down-regulated Na+/K(+)-ATPase involved // Toxicol. Lett. – 2008. – Vol. 176 (3). – P. 188–197.


10. Liang G.H., Jarlebark L., Ulfendahl M., Moore E.J. Mercury (Hg2+) suppression of potassium currents of outer hair cells // Neurotoxicol. Teratol. – 2003. – Vol. 25. – P. 349–359.


11. Shafer T.J., Meacham C.A., Barone S.Jr. Effects of prolonged exposure to nanomolar concentrations of methylmercury on voltage-sensitive sodium and calcium currents in PC12 cells // Brain Res. Dev. Brain Res. – 2002. – Vol. 136. – P. 151–164.


12. Sirois J.E., Atchison W.D. Methylmercury affects multiple subtypes of calcium channels in rat cerebellar granule cells // Toxicol. Appl. Pharmacol. – 2000. – Vol. 167. – P. 1–11.


13. Tarabová B., Kurejová M., Sulová Z. et al. Inorganic mercury and methylmercury inhibit the Cav3.1 channel expressed in human embryonic kidney 293 cells by different mechanisms // J. Pharmacol. Exp. Ther. – 2006. – Vol. 317 (1). – P. 418–427.


14. Yin X., Sun J.Z., Mei Y. et al. Effect of Hg2+ on voltage-dependent calcium channels and intracellular free calcium in trigeminal ganglion neurons of rats // Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. – 2008. – Vol. 26 (9). – P. 542–545.


15. Vislobokov A.I., Ignatov U.D., Galenko-Yaroshevskii P.A., i dr. Membranotropnoe deistvie farmakologicheskih sredstv. – Sankt-Peterbyrg – Krasnodar: Prosveshenie-Ug. – 2010. – 528 s.


16. Vislobokov A.I., Shabanov P.D. Kletochnie i molekylyarnie mehanizmi deistviya lekarstv. – Seriya: Citofarmakologiya. T. 2. – SPb.: Inform-Navigator, 2014. – 624 s.


17. Tolkynov U.A., Sibarov D.A., Frolov D.S. Aktivnost pervichnih afferentnih neironov tonkoi kishki pri deistvii gistamina modyliryetsya defensinom HNP-1 // Sensorn. sist. – 2009. – T. 23, №1. – S.79–86.


18. R Core Team. R: A language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. – 2014. URL http://www.R-project.org/.


19. Shafer T.J. Methylmercury effects on ion channels and electrical activity in neurons: future directions // Cell. Mol. Biol. – 2000. – Vol. 46 (4). – P. 855–864.


20. Hisatome I., Kurata Y., Sasaki N. et al. Block of sodium channels by divalent mercury: role of specific cysteinyl residues in the P-loop region // Biophys. J. – 2000. – Vol. 79 (3). – P. 1336–1345.


21. Divine K.K., Ayala-Fierro F., Barber D.S., Carter D.E. Glutathione, albumin, cysteine, and cys-gly effects on toxicity and accumulation of mercuric chloride in LLC-PK1 cells // J. Toxicol. Environ. Health A. – 1999. – Vol. 57 (7). – P. 489–505.


22. Skylskii I.A., Malov A.M., Glazynov V.V. Obnaryjenie perehoda ot elektrogennogo k elektroneitralnomy rejimy v rabote Na/K-nasosa neironov molluska Planorbarius corneus pri ponijenii temperatyri. Dokladi AN SSSR 1976, 231, № 4 SS. 1014 -1017.


23. Kamath S.U., Pemiah B., Sekar R.K. et al. Mercury-based traditional herbo-metallic preparations: a toxicological perspective // Arch. Toxicol. – 2012. – Vol. 86 (6). – P. 831–838.



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