1Federal state of military educational institutions of higher education «Military Medical Academy named S. M. Kirov» Ministry of Defence Russian Federation (Military Medical Academy named S. M. Kirov) 194044, Russia, St. Petersburg, Ac. Lebedev Street, 6
2National research university information technologies, mechanics and optics, St.-Petersburg
The method of atomic force microscopy studies red blood cells (RBCs) of structure, subjected cryopreservation with use of high concentration of glycerine. It is established, that the given way of freezing has ambiguous influence оn structure red blood cells in an end concentrates RBCs. According to atomic force microscopy (AFM-scanning) in red blood cells suspensions comes to light, at least, two subpopulations of cages. The first is red blood cells, adsorbing glycerine. The second – red blood cells with relative a free surface from glycerine.
Red blood cells, adsorbing glycerine are exposed piknosis to transformation, being compressed, mainly, on a short axis and height of disks. At the same time red blood cells with rather pure surface, on the contrary, increase in volume and their linear sizes exceed control values. Glycerine is adsorbed in the form of enough large particles which sizes reach 1,5 microns. Particles are grouped in characteristic branchyng structures which settle down not only on a surface red blood cells, but also on a substrate. Тhe part red blood cells adsorbs and strongly enough keeps glycerine оn а surface of cytoplasmatic membranes. Red blood cells which during technological washing up had time to be released from glycerine, open membranes a time that increases speed transmembranes маssexchange, and promotes restoration of key functions cellular cage body. Red blood cells which during technological washing uр had time to bе released from glycerine, open membranes а porous, that increases speed exchange through membranes, but promotes development of an endocellular hypostasis. Тhus, use of high concentration of glycerine for cryopreservation demands optimisation of technology washing of clearing suspensions of red blood cells from residual glycerine.
atomic force microscopy, red blood cells, cryopreservation, glycerine, water, а porous, cytoplasmatic membranes.
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