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Vol. 14, Art. 34 (pp. 400-410)    |    2013       

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The significant variable regions of hemoglobin β-chain aminoacid sequences of primates

Kostetsky P.V.

FGBUN Institute of Bioorganic Chemistry. Shemyakin and Ovchinnikov Russian Academy of Sciences

Brief summary

The aligned hemoglobin beta-chains (Hbβ) aminoacid sequences (АА) of 18 Primates species (12 Haplorrhini and 6 Strepsirrhini) were compared to locate the variable positions and regions of homologous amino acid sequences. It was found that this group of 18 Hbβ-sequences has 45 variable position, but the pairwise comparison gives maximum 32 AA-changes. Three variable regions 1-14, 51-59, 117-136 have 27 variable positions (11, 6 and 10). Using the great number of artificial homologous aminoacid sequences families - randomly permuted columns of Hbβ-sequences - was possible to show that the presence of variable regions is not accidental. The probability of the by chance appearance of the region with 11 variable positions among 14 position is very low (p<0.01). But each of the other two regions is significant (p<0.02). only as satellite of the more variable region 1-14. The location and pecularities of variable regions of Strepsirrhini and Haplorrhini Hbβ-sequences are different. The variable region 117-136 contains 10 variable position of Strepsirrhini Hbβ-sequences and only 2 variable position of Haplorrhini Hbβ-sequences. This significant difference gives possibility to predict taxon-specific peptide structure for group of Haplorrhini Hbβ-sequences – 113-124 CVLAHHFGKEFT.


Key words

variability profile, hemoglobin, probability, Рrimates.

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