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Vol. 13, Art. 77 (pp. 923-934)    |    2012       

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The possibility to application of method chemiluminescence in estimation of functional state polymorphonuclear leukocytes of blood from patients with different current of acute destructive pancreatitis

E.A.Usanova1, S.V.Chausova1, E.E. Arutyunova1, O.Yu.Filatov2, Yu.V.Balyakin1

1 The Russian National Research Medical University, Russia, Moskow

2 Moscow State Medical and Dental University, Russia, Moscow

Brief summary

In this article estimated were the fagocitic, NADPH-oxidase activity and priming effect at the polymorphonuclear leukocytes of blood under the effect of Klebsiella pneumoniae, Staphylococcus aureus complex antigen preparations with the patients having aseptic or infected pancreonecrosis. The taking away of patient\\\'s blood was performed on 1-st, 3-rd, 7-th, 14-th day of a stationary treatment by the methods of luminol and lucigenin amplified chemiluminescence. On the basic of the obtained results it has been found out that under infection of a pancreonecrosis the infringement of oxidation metabolism at polymorphonuclear leukocytes has been observed even in the early periods of disease, which is manifested in the lowering of fagocitic and NADPH-oxidase activity of polymorphonuclear leukocytes as compared to the patients with aseptic pancreonecrosis. The ability of polymorphonuclear leukocytes for primining by the complex antigen preparations of bacteria, participant in bacterial translocation under pancreonecrosis, is also reduced with these patients.


Key words

chemiluminescence, polymorphonuclear leukocytes, acute pancreatitis, pancreonecrosis, infected pancreonecrosis, priming

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