Опубликовать статью

Информационное партнерство

Инфоресурсы

Контакты

«

Vol. 13, Art. 43 (pp. 538-546)    |    2012       

»

The research hemorheological activity of hybrid macromolecular compounds on the model of blood hyperviscosity in vitro

Popova E.V., Aliev O.I., * Domnina N.S., * Sergeeva O.Y., Plotnikov M.B.

Research Institution of Pharmacology, Scientific Center of Russian Academy of Medical Sciences

Brief summary

Screening of hybrid macromolecular compounds based on hydrophilic polymers (dextran (40 kDa), hydroxyethylated starch (200 kDa) and polyethyleneglycol (20 kDa) containing fragments of sterically hindered phenols with chemically grafted polymer chains has been performed on the model of blood hyperviscosity in vitro. This work demonstrates the results of an experimental study evaluating effects of hybrid macromolecular compounds (in the end concentrations 10-5 и 10-6 g/ml of blood) whole blood hyperviscosity and erythrocyte aggregation. Substance with higher hemorheological activity is determined (О-(3-(3,5-d-tert-butyl-4-hydroxyphenyl)propenyl)-(1->6)-α-D-glucan). Was demonstrated that the hemorheological activity of hybrid macromolecular compounds depends on molecular weight of polymer. Compounds synthesized on the basis of dextran showed high positive effect on rheological properties of blood, on the basis of hydroxyethylated starch – the least influence. Polymers of hybrid macromolecular compounds in the end concentrations 10-5 и 10-6 g/ml of blood showed noneffect on the hemorheological parameters.


Key words

hybrid macromolecular compounds, red blood cell aggregation, the model of blood hyperviscosity

Reference list

1. Arefev D.V., Belostockaya I.S., Voleva V.B. i dr. Gibridnie makromolekylyarnie antioksidanti na osnove gidrofilnih polimerov i prostranstvenno-zatrydnennih fenolov // Izv. RAN. Ser. him. – 2007. – T. 4. – S. 751–760.


2. Arefev D.V., Domnina N.S., Komarova E.A. i dr. Sintez i antiradikalnaya aktivnost konugatov dekstrana i 2-(4-gidroksi-3,5- ditret.bytilfenil)propionovoi kisloti // Jyrn. prikl. himii. – 1999. – T. 72, vip. 4. – S. 670–673.


3. Belostockaya I.S., Voleva V.B., Domnina N.S. i dr. Sintez i svoistva makromolekylyarnih efirov karboksizameshennih proizvodnih prostranstvenno- zatrydnennih fenolov. // JOrH, 2010. – T. 46, № 11.– S. 1646–1651.


4. Gabrielyan E.S., Akopov S.E. Kletki krovi i krovoobrashenie. Erevan: Aiastan, 1985.– 400 s.


5. Domnina N.S., Cergeeva O.U., Hrystaleva R.S. i dr. Gibridnie makromolekylyarnie fenolnie antioksidanti. Svoistva i primenenie v medicine //Fenolnie soedineniya: fyndamentalnie i prikladnie aspekti, izd-vo Naychnii mir», 2010. – ASBM. – S. 45–53.


6. Lapotnikov V.A., Moiseev S.I. Mikrocirkylyatornii gemostaz i reologiya krovi pri perifericheskom i koronarnom ateroskleroze // Vrach. delo. – 1988. – № 4. – S. 60–63.


7. Lusov V.A., Beloysov U.B. Rol gemostaza i reologii krovi v patogeneze ishemicheskoi bolezni serdca // Kardiologiya. – 1986. – № 5. – S. 8–14.


8. Myravev A.V., Cheporov S.V. Gemoreologiya (eksperimentalnie i klinicheskie aspekti reologii krovi). – Yaroslavl: Izd-vo YaGPY, 2009. – 178 s.


9. Parfenov A.S., Peshkov A.V., Dobrovolskii N.A. Opredelenie reologicheskih svoistv krovi: Metod. rekomendacii. – M., 1994. – 15 s.


10. Patent RF № 2273483. Domnina N.S., Hrystaleva R.S., Sergeeva O.U. i dr. Vodorastvorimii polimernii antioksidant, plazmozamenitel s antiokislitelnoi i antiradikalnoi aktivnostu (varianti) i sposob podderjaniya yrovnya arterialnogo davleniya i processov antioksidantnoi zashiti v organizme pri ostroi krovopotere.


11. Plotnikov M.B., Aliev O.I., Maslov M.U. i dr. (Plotnikov M.B., Aliev O.I., Maslov M.Yu. et al.). Correction of the high blood viscosity syndrome by a mixture of diquertin and ascorbic acid in vitro and in vivo. – Phytother. Res. – 2003. – Vol. 17. – P. 276–278.


12. Plotnikov M.B., Aliev O.I., Popel F.V. Modifikaciya mikrokolorimetra MKMF-1 dlya registracii agregacii eritrocitov // Klin. lab. diagnostika. 1995. – № 3. – S. 457 –458.


13. Pokalev G.M., Kitaeva N.D., Shabanov V.A., Levin G.Ya. Mikrocirkylyaciya i reologiya serdechno-sosydistih zabolevanii: fiziologicheskie, klinicheskie i farmakologicheskie aspekti // Kardiologiya. – 1983. – № 11. – S. 89–92.


14. Savenkov M.P. Diagnostika i farmakologicheskaya korrekciya reologicheskih svoistv krovi y bolnih ishemicheskoi boleznu serdca: Avtoref. dis. … d-ra med. nayk. – M., 1988. – 33 s.


15. Sergeeva O.U., Arefev D.V., Domnina N.S., Komarova E.A. Sintez i antiokislitelnie svoistva konugatov dekstrana i 4-(4-gidroksi-3,5-di-tret.bytil-benziliden)-2-fenil-4,5digidroksazol-5-ona. // Jyrn. prikl. himii. 2005. – T. 78. – 6. – S. 962.


16. Berman W. Jr., Berman N., Pathak D., Wood S.C. Effects of pentoxifylline (Trental) on blood flow, viscosity, and oxygen transport in young adults with inoperable cyanotic congenital heart disease // Pediatr. Cardiol. – 1994. – Vol. 15, Suppl. 2. – P. 66–70.