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Vol. 12, Art. 8 (pp. 82-92)    |    jan 2011       

№ гос. регистрации: 0421100002\0008

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The use immune enzyme assay for definition of cell coexpression antigenes at carrying out of the analysis by means of immunological biochips

¹A.V. Shishkin, ¹N.G. Ovchinina, ²S.S. Bessmeltsev

1- Izhevsk State Medical Academy, Izhevsk

2- Russian Research Institute of Hematology and Transfusiology, St- Petersburg

Brief summary

The analysis by means of immunological biochips allows to define simultaneously on different cells of superficial antigens. However on each separately taken cell only one antigen upon its linkage in this or that site of the biochip with immobilised antibodies can be defined only. For definition coexpression of several antigens earlier used the additional processing of the biochip with stained antibodies. In the present work possibility of use for this purpose of antibodies, linked with enzyme molecules is shown. Such approach has advantages to carrying out of additional morphological research of the cells connected with the biochip.


Key words

immunological biochips, surface cell antigens, immunomorphological cell analysis.

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